Extending snBench to Support Hierarchical and Configurable Scheduling

It is useful in systems that must support multiple applications with various temporal requirements to allow application-specific policies to manage resources accordingly. However, there is a tension between this goal and the desire to control and police possibly malicious programs. The Java-based Sensor Execution Environment (SXE) in snBench presents a situation where such considerations add value to the system. Multiple applications can be run by multiple users with varied temporal requirements, some Real-Time and others best effort. This paper outlines and documents an implementation of a hierarchical and configurable scheduling system with which different applications can be executed using application-specific scheduling policies. Concurrently the system administrator can define fairness policies between applications that are imposed upon the system. Additionally, to ensure forward progress of system execution in the face of malicious or malformed user programs, an infrastructure for execution using multiple threads is described

Restoration of Altered MicroRNA Expression in the Ischemic Heart with Resveratrol

Resveratrol, a constituent of red wine, is important for cardioprotection. MicroRNAs are known regulators for genes involved in resveratrol-mediated cardiac remodeling and the regulatory pathway involving microRNA has not been studied so far.We explored the cardioprotection by resveratrol in ischemia/reperfusion model of rat and determined cardiac functions. miRNA profile was determined from isolated RNA using quantitative Real-time PCR based array. Systemic analyses of miRNA array and theirs targets were determined using a number of computational approaches.Cardioprotection by resveratrol and its derivative in ischemia/reperfusion [I/R] rat model was examined with miRNA expression profile. Unique expression pattern were found for each sample, particularly with resveratrol [pure compound] and longevinex [commercial resveratrol formulation] pretreated hearts. Longevinex and resveratrol pretreatment modulates the expression pattern of miRNAs close to the control level based on PCA analyses. Differential expression was observed in over 25 miRNAs, some of them, such as miR-21 were previously implicated in cardiac remodeling. The target genes for the differentially expressed miRNA include genes of various molecular function such as metal ion binding, sodium-potassium ion, transcription factors, which may play key role in reducing I/R injury.Rats pretreated with resveratrol for 3 weeks leads to significant cardioprotection against ischemia/reperfusion injury. A unique signature of miRNA profile is observed in control heart pretreated with resveratrol or longevinex. We have determined specific group of miRNA in heart that have altered during IR injuries. Most of those altered microRNA expressions modulated close to their basal level in resveratrol or longevinex treated I/R mice

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Identification of a Novel Complex between the Nucleoprotein and PA(1–27) of Influenza A Virus Polymerase

The structure of the ribonucleoprotein complexes is crucial to viral transcription and replication of influenza virus, but association of the nucleoprotein (NP) with the polymerase remains to be characterized at the molecular level. Here, we identify a peptide of the polymerase acidic subunit PA(1–27) that associates with NP. Docking and molecular dynamics simulations suggest a similar NP binding site with PA(1–27) and PA(1–186). The PA(1–27)–NP complex is characterized by surface plasmon resonance and fluorescence using recombinant NP proteins and by pull-down assays in infected cells. The PA(1–27)–NP complex may have a role in the final steps of transcription and replication

Design, synthesis and biological evaluation of vortioxetine derivatives as new COX-1/2 inhibitors in human monocytes.

In order to identify a suitable alternative to non-steroidal anti-inflammatory drugs (NSAIDs) we aimed to de- velop derivatives of vortioxetine, a multimodal anti-depressive drug that has been shownpreviously to be en- dowed withanti-inflammatory activity in human monocytes/macrophages. Vortioxetine (1) was synthesized in good yield and different alkyl and aryl derivatives were prepared based on their structural diversity and easy availability. The compounds were tested on human monocytes isolated from healthy donors for theireffect on superoxide anion production and cytokine gene expression, and for COX-1/2 gene expression and activity modulation. Moreover, a docking study was performed to predict the interactions between the synthesized compounds and COX-1 and COX-2. Correlating experimental biological data to the molecular modelling studies, it emerged that among the novel compounds, 6 was endowed of antioxidant and anti-COX-1 activity, vortiox- etine and 3 were good antioxidants and mild anti-COX-1/2 inhibitors, while 7 was a good anti-COX-1/2 inhibitor and 11 was more specific versus COX-2

Effects of resveratrol and longevinex on global miRNA expression.

<p>(A) Box-Whisker Plot of miRNA array where outliers were shown outside the black bar and excluded for analysis. The data (Ct values) were normalized based on endogeneous genes. (B) Profile plot of miRNA array filtered on expression (20.0–100.0)th Percentile in the raw data which were normalized based on endogeneous genes. BL: Baseline; I/R2h: Ischemia for 30 min and 2 h reperfusion; VEH: Vehicle, RESV: Resveratrol; LONG: Longevinex.</p

Effects of resveratrol and longevinex on blood flow, LVDP, dp/dt_max, infarct size and apoptosis.

<p>(A) Coronary flow, aortic flow and LVDP were estimated at baseline and at the indicated times of reperfusion. Infarct size and apoptosis were measured at the end of two hours of reperfusion. Results are expressed as Means±SEM of six animals per group. *p<0.05 vs. Vehicle (VEH). # p,0.05 vs corresponding I/R. BL: Baseline; I/R1h: Ischemia for 30 min and 1 h reperfusion; I/R2h: Ischemia for 30 min and 2 h reperfusion; RESV: Resveratrol; LONG: Longevinex.</p

Effects of resveratrol and longevinex on miRNA expression pattern.

<p>(A) Correlation of miRNA expressions between basal level and IR control heart using scatter plot: Few miRNA expressions were selected for display as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015705#pone-0015705-t001" target="_blank">table 1</a>. (B) Heatmap for cluster analyses of differentially expressed miRNA among samples: Each miRNA was represented as single bar based from their Ct values and color coding was shown below with a gradient from blue (negative and lowest Ct values) to red (positive and highest Ct values). miRNAs not detected were shown as black bars. Each column was represented sample indicated on top. (C) Principal component analyses of all samples. This multivariate analysis demonstrated the proximity of longivinex and resveratrol treated IR samples to the control (vehicle) samples. BL: Baseline; IR: Ischemia for 30 min and 2 h reperfusion; VEH: Vehicle, RESV: Resveratrol; LONG: Longevinex.</p

Effects of resveratrol and longevinex on phosphorylation of ERK1/2 and p38 MAPK.

<p>(A) Ratio of ERK1/2 phosphorylation to total ERK1/2 were plotted in samples as indicated. (B) Ratio of p38 MAPK phosphorylation to total p38 MAPK were plotted in samples as described. Results are expressed as Means±SEM of six animals per group. *p<0.05 vs. Vehicle (VEH). # p<0.05 vs corresponding I/R. BL: Baseline; I/R2h: Ischemia for 30 min and 2 h reperfusion; RESV: Resveratrol; LONG: Longevinex.</p